Improvement of procedure for casing treatment with a bacillus subtilis Ch-13-based biofungicide to control green mould disease of mushrooms

A previous study had confirmed good selective features of a biofungicide based on Bacillus subtilis Ch-13 regarding green mould disease control and increase in white button mushroom (Agaricus bisporus) production. The recommended application rate the three split doses (30 + 15 ml m-2) enhanced yield by 8.41% (Trichoderma aggressivum) 53.57%. different procedure B. was then further investigated, involving six (6 ? 10 same total dose 60 which previously tested doses. impact fruiting body its effectiveness were evaluated artificially infected with Trichoderma aggressivum f. europaeum experimental growing room (in vivo). No statistically significant differences efficacy found between fungicide prochloraz (71.08%) applied either (63.05%) or (58.43%). As for fungicide/biofungicide positive production, no detected among treatments. Ch-13-based may be doses, as well applications, depending watering picking schedule during cultivation, both schemes showed satisfactory effects yield.